Bioassay

Classical bioassays may be categorized into in-vivo and in-vitro assays. In-vitro bioassays are typically based upon immortalized cell lines, which show a quantifiable response after stimulation with the target drug or therapeutic agent.  

VelaLabs has extensive knowledge of the scientific principles relevant to in-vitro bioassay analysis of proteins. Typical bioassays employed at VelaLabs are (anti)-proliferation assays based on different read-outs (fluorescence, luminescence), and various antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) assays.

Cell proliferation is an essential mechanism for normal tissue development, regeneration and renewal. ADCC is a cell-mediated immune defense whereby an effector cell of the immune system actively lyses a target cell, whose membrane-surface antigens have been bound by specific antibodies. CDC is a function of the complement system that kills pathogens by damaging their membranes without the involvement of antibodies or cells of the immune system. 

Other examples in our portfolio are varieties of signaling and phosphorylation assays or functional cellular tests based on cyclic AMP.

VelaLabs has a long-term experience with different types of potency assays used for the investigation of mode-of-action of a variety of molecules. Other examples for bioassays are reporter gene assays and blood borne assays such as B-cell depletion or IFNγ release.

Besides such routinely employed bioassays, VelaLabs has the technological resources and the experience to custom design the optimal bioassay for your product. The company has repeatedly proven to be able to solve specific challenges of assay developments in a great number of clinical indications and types of development products.